Job Description
Become your most extraordinary self! Our team is intensely proud to be a major provider of services and products to the drug discovery research industry. Join Eurofins Panlabs, part of the Eurofins Discovery ™ group of companies serving serving global clients in the Pharma and Biotech industries. Work alongside industry experts and collaborate with team members to directly and positively impact human health worldwide!
We have a philosophy to support work life balance, career progression opportunities and offer many benefits such as formal mentoring programs, tuition reimbursement, extensive career training programs, competitive health benefits, paid time off and 10 annual paid holidays.
The Associate Scientist, Molecular Biology and Assay Development, is an integral member of the Eurofins DiscoverX R&D Department. They will support all departmental molecular biology and cell line development activities by executing molecular cloning of expression vectors, characterization / validation of cell lines and gene delivery to cells using viral and other methods. They will also participate in generating cell lines and/or running cell-based assays using various technologies, including Eurofins DiscoverX’s Enzyme Fragment Complementation (EFC).
Essential Duties and Responsibilities:
- Actively maintain the Eurofins DiscoverX plasmid collection including fulfilling requests for plasmids and generating additional quantities of plasmids by performing DNA preps (in-house or via out-sourcing).
- Design and generate mammalian expression constructs: identify the correct gene sequence (according to the workplan); optimize the DNA sequence (codon usage) and confirm in-frame fusion with tags (if applicable).
- Use bioinformatics tools (including SnapGene, NCBI databases, etc) for executing and confirming cloning projects.
- Routinely submit samples to, and evaluate data returned by, our external DNA sequencing partner
- Perform PCR on genomic DNA from cell lines to confirm stable integration of genes and quantitative PCR to confirm gene expression.
- Perform RNA extraction and quantify gene expression by qRT-PCR.
- Generate Biosafety Documentation.
- Perform routine cell culture, as well as stable pool and clone development of engineered cells.
- Perform characterization of engineered cell lines using molecular, biochemical, and cell biology techniques, including flow cytometry, qPCR, Western blotting, immunofluorescence or other functional readouts.
- Perform cell-based assays using established Eurofins DiscoverX assays to test activity of client molecules and report results to manager in appropriate format(s).
- Accurately prepares and presents experimental data at regular group meetings.
- Works diligently to meet product development goals set by management team.
- Provides support functions to the R&D team such as ordering and maintenance of reagents/supplies/equipment, maintaining accurate notes and records, and effective communication.
- Performs all duties in compliance with all company procedures and regulations.
- Ability to multitask and communicate effectively to ensure orderly completion and transfer of plasmids to downstream assay development projects.
- Maintains good documentation of cloning and laboratory work using various internal databases and electronic lab notebook.